Polysome profiling and cancer
WebJan 1, 2024 · Polysome-profiling of bio-banked breast cancer tissues using optimized non-linear gradients and smartSeq2 To comprehensively evaluate the non-linear sucrose … WebGlioblastoma (GBM) is one of the most aggressive cancers, with median survival of less than 2 years. Despite of considerable advance in molecular classification of GBMs, no improvements in therapy have been described. The scenario is further complicated by tumor heterogeneity and the relationship among genetic, transcriptional and functional findings. …
Polysome profiling and cancer
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WebContributed to four years of grant renewal for Dr. Blackshear’s clinical research laboratory by NIH with establishment of polysome profiling methodologies for mouse fibroblast cells and ... WebJan 7, 2024 · Circular RNAs (circRNAs) are classified as noncoding RNAs because they are devoid of a 5’ end cap and a 3’ end poly (A) tail necessary for cap-dependent translation. However, increasing numbers of translated circRNAs identified through high-throughput RNA sequencing overlapping with polysome profiling indicate that this rule is being broken. …
WebROYGBV colors (with dashed lines as boundaries) indicate the polysome fractions that were combined before 3′-seq. (C,E) 3′-seq from polysome profiling from 24-h PHS testes, plotting relative level of the long 3′ UTR isoform detected across the different polysome fractions (in each column) for genes that undergo the stage-specific 3′ UTR shortening due to APA (in … WebCancer stem cell biology / characterisation • Molecular Biology DNA/RNA techniques: cloning, qPCR, Northern blot, RNA-IP, oligo-dt mRNA pull-down, polysome profiling, cDNA/miRNA microarray, in vitro translation, mRNA protein interaction - UV crosslinking / filter binding assay, RNA modifications • Protein Biochemistry
WebExploring the field of epitranscriptomics and its implications in human cancer cell lines to identify potential drug targets. • Generated NSUN2 KO human cancer cell lines using CRISPR- Cas9. • Performed downstream analysis of NSUN2 KO cells (polysome and ribosome profiling and sequencing) and I am currently working on the functional validation. WebFeb 1, 2024 · Polysome profiling has been developed to infer the translational status of a specific mRNA species or to analyze the translatome, i.e. the subset of mRNAs actively translated in a cell. Polysome profiling is especially suitable for emergent model organisms for which genomic data are limited. In this paper, we describe an optimized protocol for ...
WebAug 10, 2024 · For example, polysome profiling has recently become a popular technique for the analysis of the ‘translatome’, that is the set of mRNA species being actively translated …
WebDec 2, 2024 · We next explored the general effect of knocking down FTO in Hela and SiHa cells, as revealed by polysome profiling, the global translation of FTO deficient cervical cancer cells was comparable to FTO competent controls (Fig. 5c), however, when we evaluated the translation of E2F1 and Myc specifically, we found the translation efficiency … open invite meaningWebMay 17, 2014 · An example of applying the polysome fractionation method to investigate the role of mTOR signaling in mediating the effects of insulin on mRNA translation is presented. To this end, MCF7 human breast cancer cells were maintained in low serum and then stimulated with insulin alone or in combination with the active-site mTOR inhibitor Torin1. open invoice for daceasy query builderWebSep 18, 2024 · Polysome profiling analysis of the early passage Eμ-Myc lymphoma “CX” cell lines and investigating potential roles of RAPGEF3 (EPAC1) and RAPGEF4 (EPAC2) in human blood cancers A. Enrichment analysis by GeneGO MetaCore ® of the polysomal RNA-seq data comparing CX-5461-resistant (CX) and drug-naïve cells (CTRL; n = 3; false discovery … open invoice and oildexWebDec 10, 2024 · Polysome profiling measures ribosome density of each mRNA , ... This study is supported by the PEW Charitable Trusts, Cancer Research Institute, National Institute of Health (R01AI087634, R01AI089854, RC1CA146299, R56AI110403, and R01AI121155 to C.X.). Author information. open invitation 歌詞WebSep 16, 2024 · Polysome profiling of cells treated with three hypothetical translation inhibitors is shown in the plots below. These three inhibitors are: (i) ... The cancer cells differentiated into a mesenchymal phenotype and grew in fresh culture medium containing inhibitors of epithelial-to-mesenchymal transition (EMT). open invoice customer supportWebFeb 19, 2024 · Polysome profiling is a widely used method to monitor the translation status of mRNAs. Although it is theoretically a simple technique, it is labor intensive. Repetitive polysome fractionation rapidly generates a large number of samples to be handled in the downstream processes of protein elimination, RNA extraction and quantification. Here, we … openinvoice onboardWebLearned many techniques such as Cloning, Western blotting, Luciferase Assay, Polysome profiling, Plasmid and Genomic DNA Isolation, PAGE, Polymerase Chain Reaction, Site-directed ... Worked in Gamma Radiobiology lab on the project titled,"Bone marrow cancer treatment through Emergency drug testing(IP,IV) in mice by bone marrow ... ipad air 2 restore mode