Phosphoserine ab
WebJan 2, 2007 · In the case of phosphoserine Ab, blots were blocked for 1 h at room temperature, probed (1:1000) overnight at 4 °C and incubated with HRP-conjugated goat anti-rabbit Ab (1:2000) for 1 h at room temperature. Signal was developed using enhanced chemiluminescence Western blot detection reagents (Pierce).3. Results3.1. WebFeb 13, 2015 · Immunoblot data showed that the phosphopeptide but not the peptide without the phosphoserine eluted the AKB1 from the co-IP complexes efficiently, ... About 5 µl of each eluates were subjected to WB using anti-AKB1 Ab (left panel) and kinase assay using PKC Pep-Tag assay kit substrate (right panel). ...
Phosphoserine ab
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WebThe Phospho- (Ser/Thr) Phe Antibody is a powerful tool for discovery of new proteins containing this important regulatory motif. Pinna, L.A. and Ruzzene, M. (1996) Biochim Biophys Acta 1314, 191-225. Yaffe, M.B. and Elia, A.E. … WebThe anti-phosphoserine and anti-phosphothreonine Abs are from Millipore, Cat# AB1603 & AB1607 respectively. Protocol 2 - Immunoprecipitation using Phosphotyrosine Antibody Reagents Use as indicated at 5 µl per IP reaction (200-500 µg total lysate per IP), sufficient for approximately 40 IP assays. Method 1. Add 5 µl of anti-phosphotyrosine (Cat.
WebMonoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Ser65 of human Ubiquitin protein. Background Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. WebAug 15, 2005 · Bands at approximately 980 and 1080cm(-1) in solution Raman spectra of phosphoserine and phosphothreonine are assigned to the monobasic and dibasic phosphate groups, respectively. Calibrated Raman peak area ratio measurements, performed as a function of pH, are used to determine the corresponding pKa values of 5.6 …
WebSynthetic peptide containing phosphorylated serine Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide Preparation The antibody was purified by affinity chromatography. Concentration 0.5 mg/mL Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. Application WB - Quality tested WebOct 15, 2024 · The variables dipm and dften derived from ab initio quantum chemical calculations as described above correlate differently ... O'Donoghue P. Hohn MJ, et al. (2006) RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea. Proc Nat Acad Sci USA 103 (50): 18923–18927. 10.1073/pnas.0609703104 …
WebPhosphoserine/threonine Antibody (MA5-38234) in WB. Western blot analysis of extracts of Jurkat cells using Phosphoserine/threonine Monoclonal Antibody ( Product # MA5-38234 ). Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at …
WebSpecificity. This antibody detects free and conjugated phosphoserine as well as phosphoserine in modified cellular proteins. It does not cross react with … bishop ncis wikiWebFeb 26, 2024 · Anti-p-Ser/Phosphoserine Antibody (16B4) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation). Mouse … dark orange couch coverWebPhosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. bishop ndingi primary school moloWebThe protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, … bishop neal buckonWebAnti-phospho Serine Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Immunology, Cancer research and Signal Transduction research. Application Note Phosphoserine Antibody is tested for use in ELISA, western blotting, Immunohistochemistry, and immunoprecipitation assays. dark orange kitchen with black appliancesWebFeb 23, 2024 · Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a … bishop neal robersonWebPhosphoserine (abbreviated as SEP or J) is an ester of serine and phosphoric acid. Phosphoserine is a component of many proteins as the result of posttranslational … bishop neal j. buckon